MM:  Michaelis-Menten Enzyme Kinetics Software

MM is an MS Windows application designed to explore the Michaelis-Menten enzyme kinetics model through a laboratory simulation. The lab instructions are part of the program, though one can read the tutorial in the file MM_Tutorial.doc. The tutorial allows students to produce graphs of the concentrations of free enzyme, enzyme-substrate complex, and product versus time in order to learn about the approach to steady state.  By using a range of substrate concentrations and rate constants, students are able to simulate enzyme kinetics and produce Lineweaver-Burk plots.  The tutorial is intended for use in junior/senior level undergraduate biochemistry courses.

Download/Install Instructions:

Download the MMSETUP.ZIP (or MM12.ZIP) file and unzip it on your desktop PC.  Run the Setup file and it will install the needed files on to your PC.

Version 1.2 (Jan 2006)

Version 1.1: Download MM (2000-2005)

Additional Documentation:

• A Class-Tested Lab Tutorial:                            MM_Tutorial.doc
• Class Survey Instrument:                                 MM_Survey.doc
• MM Guide:                                                     Guide.doc
• Briggs-Haldane System of ODEs:                     MM_Theory.doc
• Mathcad implementation                                  kinetics02.mcd
• Flash Tutorial (2005): We have provided a Flash Tutorial for seeing how this program (Version 1.1) is used. It is about 18.6 MB. You can also download the Flash File by right-clicking and saving to your machine locally. [You might also try the EXE file.]  You will need the Flash Player to view this tutorial.
• Paper submitted to J. Chem. Ed.

Screenshots: (Version 1.2)

Interface:

Change Parameters:

Change S[0]:

Saved Data:

Tutorial:

Guide:

MM was written by Dr. Russell L. Herman and
Kinetics02.mcd was written by Dr. Gabriel Lugo,
Department of Mathematics and Statistics,
University of North Carolina at Wilmington. © 2000, © 2005, © 2006
The Lab Tutorial was written and class tested by Dr. Christopher J. Halkides
Department of Chemistry,
University of North Carolina at Wilmington.

For more information, contact Dr. Russell L. Herman at hermanr@uncw.edu